Journal: Stem Cell Research & Therapy

Article Title: Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study

doi: 10.1186/s13287-021-02291-z

Figure Lengend Snippet: Primers used for RT-PCR

Article Snippet: Immunochemical staining for C/EBP-α (Bioss, China, cat.bs1630R), PPARγ (Bioss, China, cat.bs4888R), Adiponectin (Bioss, China, cat.bs0471R), and CD31 (Sino Biological, China, cat.50408-T16) was performed to determine the extent of adipose tissue and blood vessel formation in new growth tissues at the 4th week.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study

doi: 10.1186/s13287-021-02291-z

Figure Lengend Snippet: The biological responses of ADSCs to sEV-LT and sEV-AT. a The uptake of sEV-LT and sEV-AT by ADSCs. ADSCs were incubated with DiO-labeled extracellular vesicles (green) and stained with phallotoxins (red). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. b Representative images from the scratch wound assay. Scale bar = 200 μm. c Quantitative analysis of cell migration in each group at 12 h and 24 h ( n = 3). d CCK-8 assay ( n = 3). e ADSCs cultured with sEV-LT and sEV-AT for 14 days, and the lipid droplets were stained with Oil Red O to determine the level of adipogenesis. ADSCs cultured with basal culture medium (negative control, NC) or adipogenic medium (ADIPO) were used as a negative and positive controls, respectively. Scale bar = 50 μm. f Quantification of the amount of Oil Red O ( n = 3). g The relative expressions of mRNA encoding C/EBPδ, PPARγ2, Adiponectin was measured by RT-PCR on day 7 after induction. Results are present as mean ± s.d. ( n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001

Article Snippet: Immunochemical staining for C/EBP-α (Bioss, China, cat.bs1630R), PPARγ (Bioss, China, cat.bs4888R), Adiponectin (Bioss, China, cat.bs0471R), and CD31 (Sino Biological, China, cat.50408-T16) was performed to determine the extent of adipose tissue and blood vessel formation in new growth tissues at the 4th week.

Techniques: Incubation, Labeling, Staining, Scratch Wound Assay Assay, Migration, CCK-8 Assay, Cell Culture, Negative Control, Reverse Transcription Polymerase Chain Reaction

Journal: Stem Cell Research & Therapy

Article Title: Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study

doi: 10.1186/s13287-021-02291-z

Figure Lengend Snippet: Immunohistochemistry of neotissue sections in the experimental and control groups at week 4 after transplantation. Representative photographs of C/EBPα, PPARγ, Adiponectin, and CD31 immunostaining in the Matrigel group, sEV-LT group, and sEV-AT group, respectively. The black arrows indicated the positive stained protein. Scale bar = 50 μm

Article Snippet: Immunochemical staining for C/EBP-α (Bioss, China, cat.bs1630R), PPARγ (Bioss, China, cat.bs4888R), Adiponectin (Bioss, China, cat.bs0471R), and CD31 (Sino Biological, China, cat.50408-T16) was performed to determine the extent of adipose tissue and blood vessel formation in new growth tissues at the 4th week.

Techniques: Immunohistochemistry, Transplantation Assay, Immunostaining, Staining